Development of Serine/Threonine Ligation (STL) and Its Applications in the Synthesis of Proteins and Cyclic Peptides — ASN Events
  1. Schedule
  2. Session 5 SP Protein Synthesis
  3. Development of Serine/Threonine Ligation (STL) and Its Applications in the Synthesis of Proteins and Cyclic Peptides

Development of Serine/Threonine Ligation (STL) and Its Applications in the Synthesis of Proteins and Cyclic Peptides (#19)

Xuechen Li 1
  1. The University of Hong Kong, Hong Kong

Chemical ligation which couples side-chain unprotected peptide segments has become an attractive tool in the convergent peptide and protein synthesis.1-2 In line with the development of peptide ligation methods, our laboratory has recently described the use of a peptidyl salicylaldehyde (SAL) ester reacting with an N-terminal serine or threonine residue followed by acidolysis to generate a natural peptidic bond at the ligation site. We have demonstrated the effectiveness of this method with the synthesis of proteins and cyclic peptides. 3-5

  1. (1) P. E. Dawson, T. W. Muir, I. Clark-Lewis, S. B. H. Kent. (1994) Science 266, 776 (2) C. F. Liu, J. P. Tam. (1994) Proc. Natl. Acad. Sci. USA 91, 6584. (3) Y. Zhang, C. Xu, H. Y. Lam, C. L. Lee, X. Li. (2013) Proc. Natl. Acad. Sci. USA. 110, 6657. (4) C. Xu, H. Y. Lam, Y. Zhang, X. Li. (2013) Chem. Commun. 49, 6200. (5) H. Y. Lam, Y. Zhang, H. Liu, J. Xu, C. T. T. Wong, C. Xu, X. Li (2013) J. Am. Chem. Soc. 135, 6272.