As advances in genome-wide sciences, the protein-detection microarrays have been promising technologies providing high-throughput detection for proteins of interest.  To realize such a practical protein-detection system, development of capturing agents is one of the important steps.  Peptides are the promising candidates for capturing agents of protein-detection chips.  Peptide de novo designing strategy for α-helix, β-sheet and β-loop enables peptide microarrays to mimic protein-protein interactions and discriminate protein structures.  Thus, we have attempted to develop designed peptide microarrays for protein detection and cell characterization.  Fluorescent peptide libraries with designed sequences for α-helix, β-sheet and β-loop were constructed, and immobilized or deposited on a spot to afford designed peptide microarrays.  Fluorescence data of protein binding were demonstrated to show color-coded patterns regarded as protein fingerprints (PFP).

Furthermore, these peptide library and microarray are also applied for cell assay systems.  A peptide library designed for α-helix was constructed by chemical synthesis. Peptides expressing a high cell-penetrating (CPP) activity were screened, and then cell types were discriminated and profiled using the cell fingerprint (CFP) with the CPP activity of the library.  Throughout these studies, it has been demonstrated that the designed peptide microarray is one of useful tools for protein and cell chip technologies.